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mammalian expression vector pfuse higg1 fc2  (InvivoGen)


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    InvivoGen mammalian expression vector pfuse higg1 fc2
    Mammalian Expression Vector Pfuse Higg1 Fc2, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pfuse+higg1+fc2+expression+vector/pFUSE-hIgG1-Fc2/10__3390_slash_ijms27041895-177-23-27
    Average 96 stars, based on 215 article reviews
    mammalian expression vector pfuse higg1 fc2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Expressing:

    Article Title: Dissecting the cytomegalovirus CC chemokine: Chemokine activity and gHgLchemokine-dependent cell tropism are independent players in CMV infection
    Article Snippet: For expression of recombinant untagged MCK2 proteins, the pCR3 vector (Invitrogen) was used. .. For expression of recombinant MCK2 human IgG1Fc fusion proteins, the pFuse-hIgG1-Fc2 expression vector (Invivogen) was used. ..

    Article Title: Identification and Comparison of the Sialic Acid-Binding Domain Characteristics of Avian Coronavirus Infectious Bronchitis Virus Spike Protein.
    Article Snippet: Infectious bronchitis virus (IBV) infections are initiated by the transmembrane spike (S) glycoprotein, which binds to host factors and fuses the viral and cell membranes.. The N-terminal domain of the S1 subunit of IBV S protein binds to sialic acids, but the precise location of the sialic acid binding domain (SABD) and the role of the SABD in IBV-infected chickens remain unclear.. Here, we identify the S1 N-terminal amino acid (aa) residues 19 to 227 (209 aa total) of IBV strains SD (GI-19) and GD (GI-7), and the corresponding region of M41 (GI-1), as the minimal SABD using truncated protein histochemistry and neuraminidase assays.

    Article Title: Fully human antibody specifically inhibiting Connexin 26
    Article Snippet: Methods: The positive sequence obtained in Example 1 (i.e., SEQ ID NO: 3) was synthesized by gene synthesis method (Nanjing GenScript Ltd.) and added with the restriction sites BamH1 and BglII at both ends of the sequence. .. The obtained nucleic acid sequence was inserted into the pFUSE-hIGg1-FC2 expression vector (Invivogen Corporation, with reference to the procedures described in the instruction manual) after enzyme digestion at the restriction sites BamH1 and BglII with reference to the procedures described in the instruction manual of NEB to obtain an eukaryotic antibody expression plasmid of Anti-Cx26-scFvII-Fc, which is a single chain antibody having an Fc segment. .. 293Fectin reagent (Invitrogen Corporation, Cat. No. 12347500) was mixed with the eukaryotic antibody expression plasmid with a volume mass ratio of 30 microliter: 15 micrograms.

    Article Title: S-Layer From Lactobacillus brevis Modulates Antigen-Presenting Cell Functions via the Mincle-Syk-Card9 Axis
    Article Snippet: .. Briefly, the cDNA encoding the extracellular part of murine Mincle was amplified by polymerase chain reaction (PCR) and was then ligated into the pFuse-hIgG1-Fc2 expression vector (Invivogen #pfuse-hg1fc2) using the following primers: Mincle-FW 5′-CCATGGGGCAGAACTTACAGCCACAT-3′ and RV 5′-AGATCTGTCCAGAGGACTTATTTCTG-3′). .. CHO-S cells were transiently transfected with the construct using MAX reagent (InvivoGen, San Diego, California, United States).

    Article Title: Human IgG Fc-engineering for enhanced plasma half-life, mucosal distribution and killing of cancer cells and bacteria
    Article Snippet: .. The HA cDNA was subcloned into the pFUSE-hIgG1-Fc2 expression vector (InvivoGen) to generate pFUSE-HA(PR8)-hIgG1-Fc2. ..

    Article Title: Inhibition of myeloid-derived suppressor cell arginase-1 production enhances T-cell-based immunotherapy against Cryptococcus neoformans infection
    Article Snippet: .. Briefly, the gene fragment of human CLEC2D C-type lectin-like domain (CTLD) was amplified from human PBMCs total cDNA and then cloned into the pFUSE-hIgG1-Fc2 expression vector (Invivogen, Catalog # pfuse-hg1fc2, Version # 06G05-MT). .. Sequenced plasmid was transfected into HEK293T-cells using Lipo2000 (Thermo Fisher Scientific, 11668019) according to the manufacturer’s instructions.

    Recombinant:

    Article Title: Dissecting the cytomegalovirus CC chemokine: Chemokine activity and gHgLchemokine-dependent cell tropism are independent players in CMV infection
    Article Snippet: For expression of recombinant untagged MCK2 proteins, the pCR3 vector (Invitrogen) was used. .. For expression of recombinant MCK2 human IgG1Fc fusion proteins, the pFuse-hIgG1-Fc2 expression vector (Invivogen) was used. ..

    Plasmid Preparation:

    Article Title: Dissecting the cytomegalovirus CC chemokine: Chemokine activity and gHgLchemokine-dependent cell tropism are independent players in CMV infection
    Article Snippet: For expression of recombinant untagged MCK2 proteins, the pCR3 vector (Invitrogen) was used. .. For expression of recombinant MCK2 human IgG1Fc fusion proteins, the pFuse-hIgG1-Fc2 expression vector (Invivogen) was used. ..

    Article Title: Identification and Comparison of the Sialic Acid-Binding Domain Characteristics of Avian Coronavirus Infectious Bronchitis Virus Spike Protein.
    Article Snippet: Infectious bronchitis virus (IBV) infections are initiated by the transmembrane spike (S) glycoprotein, which binds to host factors and fuses the viral and cell membranes.. The N-terminal domain of the S1 subunit of IBV S protein binds to sialic acids, but the precise location of the sialic acid binding domain (SABD) and the role of the SABD in IBV-infected chickens remain unclear.. Here, we identify the S1 N-terminal amino acid (aa) residues 19 to 227 (209 aa total) of IBV strains SD (GI-19) and GD (GI-7), and the corresponding region of M41 (GI-1), as the minimal SABD using truncated protein histochemistry and neuraminidase assays.

    Article Title: Fully human antibody specifically inhibiting Connexin 26
    Article Snippet: Methods: The positive sequence obtained in Example 1 (i.e., SEQ ID NO: 3) was synthesized by gene synthesis method (Nanjing GenScript Ltd.) and added with the restriction sites BamH1 and BglII at both ends of the sequence. .. The obtained nucleic acid sequence was inserted into the pFUSE-hIGg1-FC2 expression vector (Invivogen Corporation, with reference to the procedures described in the instruction manual) after enzyme digestion at the restriction sites BamH1 and BglII with reference to the procedures described in the instruction manual of NEB to obtain an eukaryotic antibody expression plasmid of Anti-Cx26-scFvII-Fc, which is a single chain antibody having an Fc segment. .. 293Fectin reagent (Invitrogen Corporation, Cat. No. 12347500) was mixed with the eukaryotic antibody expression plasmid with a volume mass ratio of 30 microliter: 15 micrograms.

    Article Title: Human IgG Fc-engineering for enhanced plasma half-life, mucosal distribution and killing of cancer cells and bacteria
    Article Snippet: .. The HA cDNA was subcloned into the pFUSE-hIgG1-Fc2 expression vector (InvivoGen) to generate pFUSE-HA(PR8)-hIgG1-Fc2. ..

    Article Title: Screening for C-type lectin receptor (CLR)/bacteria interactions using a bovine CLR-Fc fusion protein library reveals recognition of Pasteurella multocida B:2 by MICL.
    Article Snippet: To amplify the cDNA fragment of interest, the Phusion High-Fidelity DNA Polymerase (NEB) was used. .. Following amplification, the PCR products were digested with the corresponding restriction enzymes (NEB) and ligated into a pFUSE-hIgG1-Fc2 expression vector (InvivoGen) encoding the human Fc (hFc) domain. .. Sequence verification was performed by Sanger sequencing using a Mix2Seq Kit (Eurofins).

    Amplification:

    Article Title: Identification and Comparison of the Sialic Acid-Binding Domain Characteristics of Avian Coronavirus Infectious Bronchitis Virus Spike Protein.
    Article Snippet: Infectious bronchitis virus (IBV) infections are initiated by the transmembrane spike (S) glycoprotein, which binds to host factors and fuses the viral and cell membranes.. The N-terminal domain of the S1 subunit of IBV S protein binds to sialic acids, but the precise location of the sialic acid binding domain (SABD) and the role of the SABD in IBV-infected chickens remain unclear.. Here, we identify the S1 N-terminal amino acid (aa) residues 19 to 227 (209 aa total) of IBV strains SD (GI-19) and GD (GI-7), and the corresponding region of M41 (GI-1), as the minimal SABD using truncated protein histochemistry and neuraminidase assays.

    Article Title: S-Layer From Lactobacillus brevis Modulates Antigen-Presenting Cell Functions via the Mincle-Syk-Card9 Axis
    Article Snippet: .. Briefly, the cDNA encoding the extracellular part of murine Mincle was amplified by polymerase chain reaction (PCR) and was then ligated into the pFuse-hIgG1-Fc2 expression vector (Invivogen #pfuse-hg1fc2) using the following primers: Mincle-FW 5′-CCATGGGGCAGAACTTACAGCCACAT-3′ and RV 5′-AGATCTGTCCAGAGGACTTATTTCTG-3′). .. CHO-S cells were transiently transfected with the construct using MAX reagent (InvivoGen, San Diego, California, United States).

    Article Title: Inhibition of myeloid-derived suppressor cell arginase-1 production enhances T-cell-based immunotherapy against Cryptococcus neoformans infection
    Article Snippet: .. Briefly, the gene fragment of human CLEC2D C-type lectin-like domain (CTLD) was amplified from human PBMCs total cDNA and then cloned into the pFUSE-hIgG1-Fc2 expression vector (Invivogen, Catalog # pfuse-hg1fc2, Version # 06G05-MT). .. Sequenced plasmid was transfected into HEK293T-cells using Lipo2000 (Thermo Fisher Scientific, 11668019) according to the manufacturer’s instructions.

    Polymerase Chain Reaction:

    Article Title: Identification and Comparison of the Sialic Acid-Binding Domain Characteristics of Avian Coronavirus Infectious Bronchitis Virus Spike Protein.
    Article Snippet: Infectious bronchitis virus (IBV) infections are initiated by the transmembrane spike (S) glycoprotein, which binds to host factors and fuses the viral and cell membranes.. The N-terminal domain of the S1 subunit of IBV S protein binds to sialic acids, but the precise location of the sialic acid binding domain (SABD) and the role of the SABD in IBV-infected chickens remain unclear.. Here, we identify the S1 N-terminal amino acid (aa) residues 19 to 227 (209 aa total) of IBV strains SD (GI-19) and GD (GI-7), and the corresponding region of M41 (GI-1), as the minimal SABD using truncated protein histochemistry and neuraminidase assays.

    Article Title: S-Layer From Lactobacillus brevis Modulates Antigen-Presenting Cell Functions via the Mincle-Syk-Card9 Axis
    Article Snippet: .. Briefly, the cDNA encoding the extracellular part of murine Mincle was amplified by polymerase chain reaction (PCR) and was then ligated into the pFuse-hIgG1-Fc2 expression vector (Invivogen #pfuse-hg1fc2) using the following primers: Mincle-FW 5′-CCATGGGGCAGAACTTACAGCCACAT-3′ and RV 5′-AGATCTGTCCAGAGGACTTATTTCTG-3′). .. CHO-S cells were transiently transfected with the construct using MAX reagent (InvivoGen, San Diego, California, United States).

    Clone Assay:

    Article Title: The PD-L1 and TLR7 dual-targeting nanobody-drug conjugate exerts potent antitumor efficacy by orchestrating innate and adaptive immune responses
    Article Snippet: Animal care and experiments were performed in accordance with GemPharmatech and the Shanghai Institute of Materia Medica, Chinese Academy of Sciences, using protocols approved by the Institutional Laboratory Animal Care and Use Committee (IACUC). .. Genes encoding mouse PD-L1 (mouse PD-L1, amino acid residues 19-239) and human PD-L1 (amino acid residues 19-238) were cloned into pFUSE-hIgG1-Fc2 expression vector (Invivogen). ..

    Article Title: Inhibition of myeloid-derived suppressor cell arginase-1 production enhances T-cell-based immunotherapy against Cryptococcus neoformans infection
    Article Snippet: .. Briefly, the gene fragment of human CLEC2D C-type lectin-like domain (CTLD) was amplified from human PBMCs total cDNA and then cloned into the pFUSE-hIgG1-Fc2 expression vector (Invivogen, Catalog # pfuse-hg1fc2, Version # 06G05-MT). .. Sequenced plasmid was transfected into HEK293T-cells using Lipo2000 (Thermo Fisher Scientific, 11668019) according to the manufacturer’s instructions.

    Sequencing:

    Article Title: Fully human antibody specifically inhibiting Connexin 26
    Article Snippet: Methods: The positive sequence obtained in Example 1 (i.e., SEQ ID NO: 3) was synthesized by gene synthesis method (Nanjing GenScript Ltd.) and added with the restriction sites BamH1 and BglII at both ends of the sequence. .. The obtained nucleic acid sequence was inserted into the pFUSE-hIGg1-FC2 expression vector (Invivogen Corporation, with reference to the procedures described in the instruction manual) after enzyme digestion at the restriction sites BamH1 and BglII with reference to the procedures described in the instruction manual of NEB to obtain an eukaryotic antibody expression plasmid of Anti-Cx26-scFvII-Fc, which is a single chain antibody having an Fc segment. .. 293Fectin reagent (Invitrogen Corporation, Cat. No. 12347500) was mixed with the eukaryotic antibody expression plasmid with a volume mass ratio of 30 microliter: 15 micrograms.



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